Native metabolomics for mass spectrometry-based siderophore discovery

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00587971" target="_blank" >RIV/61388963:_____/24:00587971 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/bs.mie.2024.07.001" target="_blank" >https://doi.org/10.1016/bs.mie.2024.07.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/bs.mie.2024.07.001" target="_blank" >10.1016/bs.mie.2024.07.001</a>

Result language

angličtina

Original language name

Native metabolomics for mass spectrometry-based siderophore discovery

Original language description

Microorganisms, plants, and animals alike have specialized acquisition pathways for obtaining metals, with microorganisms and plants biosynthesizing and secreting small molecule natural products called siderophores and metallophores with high affinities and specificities for iron or other non-iron metals, respectively. This chapter details a novel approach to discovering metal-binding molecules, including siderophores and metallophores, from complex samples ranging from microbial supernatants to biological tissue to environmental samples. This approach, called Native Metabolomics, is a mass spectrometry method in which pH adjustment and metal infusion post-liquid chromatography are interfaced with ion identity molecular networking (IIMN). This rule-based data analysis workflow that enables the identification of metal-binding species based on defined mass (m/z) offsets with the same chromatographic profiles and retention times. Ion identity molecular networking connects compounds that are structurally similar by their fragmentation pattern and species that are ion adducts of the same compound by chromatographic shape correlations. This approach has previously revealed new insights into metal binding metabolites, including that yersiniabactin can act as a biological zincophore (in addition to its known role as a siderophore), that the recently elucidated lepotchelin natural products are cyanobacterial metallophores, and that antioxidants in traditional medicine bind iron. Native metabolomics can be conducted on any liquid chromatography-mass spectrometry system to explore the binding of any metal or multiple metals simultaneously, underscoring the potential for this method to become an essential strategy for elucidating biological metal-binding molecules.

Czech name

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Czech description

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Type

C - Chapter in a specialist book

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

Methods in Enzymology. Siderophore and Related Natural Products Biosynthesis

ISBN

978-0-443-29678-9

Number of pages of the result

36

Pages from-to

317-352

Number of pages of the book

402

Publisher name

Academic Press

Place of publication

Cambridge

UT code for WoS chapter

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