Direct detection of stringent alarmones (pp)pGpp using malachite green
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00588521" target="_blank" >RIV/61388963:_____/24:00588521 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.15698/mic2024.08.834" target="_blank" >https://doi.org/10.15698/mic2024.08.834</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15698/mic2024.08.834" target="_blank" >10.15698/mic2024.08.834</a>
Alternative languages
Result language
angličtina
Original language name
Direct detection of stringent alarmones (pp)pGpp using malachite green
Original language description
The alarmone (p)ppGpp serves as the signalling molecule for the bacterial universal stringent response and plays a crucial role in bacterial virulence, persistence, and stress adaptation. Consequently, there is a significant focus on developing new drugs that target and modulate the levels of (p)ppGpp as a potential strategy for controlling bacterial infections. However, despite the availability of various methods for detecting (p)ppGpp, a simple and straightforward detection method is needed. In this study, we demonstrated that malachite green, a well-established compound used for phosphate detection, can directly detect (p)ppGpp and its analogues esp., pGpp. By utilizing malachite green, we identified three new inhibitors of the hydrolase activity of SpoT, one of the two RelA-SpoT homolog (RSH) proteins responsible for making and hydrolyzing (p)ppGpp in Escherichia coli. . These findings highlight the convenience and practicality of malachite green, which can be widely employed in high-throughput studies to investigate (pp)pGpp in vitro and discover novel regulators of RSH proteins.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10401 - Organic chemistry
Result continuities
Project
<a href="/en/project/LX22NPO5103" target="_blank" >LX22NPO5103: National Institute of Virology and Bacteriology</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microbial cell
ISSN
2311-2638
e-ISSN
2311-2638
Volume of the periodical
11
Issue of the periodical within the volume
1
Country of publishing house
AT - AUSTRIA
Number of pages
9
Pages from-to
312-320
UT code for WoS article
001284820900001
EID of the result in the Scopus database
2-s2.0-85209735045