Global analysis by LC-MS/MS of N6-methyladenosine and inosine in mRNA reveal complex incidence

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00618830" target="_blank" >RIV/61388963:_____/25:00618830 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/25:00141605
Result on the web
<a href="http://www.rnajournal.org/cgi/doi/10.1261/rna.080324.124" target="_blank" >http://www.rnajournal.org/cgi/doi/10.1261/rna.080324.124</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1261/rna.080324.124" target="_blank" >10.1261/rna.080324.124</a>

Result language
angličtina
Original language name
Global analysis by LC-MS/MS of N6-methyladenosine and inosine in mRNA reveal complex incidence
Original language description
The precise and unambiguous detection and quantification of internal RNA modifications represents a critical step for understanding their physiological functions. The methods of direct RNA sequencing are quickly developing allowing for the precise location of internal RNA marks. This detection is, however, not quantitative and still presents detection limits. One of the biggest remaining challenges in the field is still the detection and quantification of m6A, m6Am, inosine, and m1A modifications of adenosine. The second intriguing and timely question remaining to be addressed is the extent to which individual marks are coregulated or potentially can affect each other. Here, we present a methodological approach to detect and quantify several key mRNA modifications in human total RNA and in mRNA, which is difficult to purify away from contaminating tRNA. We show that the adenosine demethylase FTO primarily targets m6Am marks in noncoding RNAs in HEK293T cells. Surprisingly, we observe little effect of FTO or ALKBH5 depletion on the m6A mRNA levels. Interestingly, the upregulation of ALKBH5 is accompanied by an increase in inosine level in overall mRNA.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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