Milligram-Scale Enzymatic Synthesis of Base-Modified DNA through Primer Extension: Polymerase Synthesis and NMR Structures of DNA Containing Phenyl-Substituted Nucleobases

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00637921" target="_blank" >RIV/61388963:_____/25:00637921 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1002/ceur.202500186" target="_blank" >https://doi.org/10.1002/ceur.202500186</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/ceur.202500186" target="_blank" >10.1002/ceur.202500186</a>

Result language

angličtina

Original language name

Milligram-Scale Enzymatic Synthesis of Base-Modified DNA through Primer Extension: Polymerase Synthesis and NMR Structures of DNA Containing Phenyl-Substituted Nucleobases

Original language description

Milligram (≈200 nmol) scale enzymatic synthesis of base-modified DNA is developed based on primer extension using easily accessible and cheap KOD (exo–) DNA polymerase with optimized protocol and streamlined isolation. The methodology is applied for synthesis of 31-mer and 18-mer DNA containing 5-phenylpyrimidine or 7-phenyl-7-deazapurine nucleobases using the corresponding phenyl-modified dNPhTPs in the PEX reactions. For the 18mers, the isolated yields are 24–61% of modified DNA samples of good purity, whereas for 31mers the samples contain minor amounts or shorter or longer impurities. The obtained amounts of modified DNA samples are sufficient for NMR structural analysis and CD spectroscopy that reveal only minor differences from B-DNA structure of the same non-modified DNA duplexes.

Czech name

—

Czech description

—

Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ChemistryEurope

ISSN

2751-4765

e-ISSN

—

Volume of the periodical

3

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

e202500186

UT code for WoS article

—

EID of the result in the Scopus database

—