Toward reproducible PETase research: A standardized workflow for reliable enzyme production and comparison
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00638493" target="_blank" >RIV/61388963:_____/25:00638493 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.pep.2025.106801" target="_blank" >https://doi.org/10.1016/j.pep.2025.106801</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.pep.2025.106801" target="_blank" >10.1016/j.pep.2025.106801</a>
Alternative languages
Result language
angličtina
Original language name
Toward reproducible PETase research: A standardized workflow for reliable enzyme production and comparison
Original language description
The enzymatic degradation of polyethylene terephthalate (PET) by PETases has gained significant attention as a potential solution for plastic waste management. However, the absence of a standardized protocol for PETase production across studies presents a challenge for consistent enzyme characterization and activity comparison. Variations in production methods, including expression systems and purification techniques, may contribute to discrepancies in reported PETase activities. Here, we present the development of a unified protocol for the production of wild-type and engineered IsPETase variants. This protocol comprises standardized expression, purification, and quality control steps to ensure reproducibility and reliability. By enabling more accurate comparisons of PETase variants and addressing inconsistencies in PETase production, this approach facilitates collaborative efforts to advance plastic degradation technologies and lays the groundwork for accelerating research in enzymatic PET degradation and its applications in plastic waste management.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Protein Expression and Purification
ISSN
1046-5928
e-ISSN
1096-0279
Volume of the periodical
236
Issue of the periodical within the volume
December
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
106801
UT code for WoS article
001564886900001
EID of the result in the Scopus database
2-s2.0-105013159537