Exploring the Impact of Palmitoylated PrRP31 and Liraglutide in Mouse Model of Obesity and MASLD

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00638937" target="_blank" >RIV/61388963:_____/25:00638937 - isvavai.cz</a>

Result on the web

<a href="https://hdl.handle.net/11104/0369482" target="_blank" >https://hdl.handle.net/11104/0369482</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Exploring the Impact of Palmitoylated PrRP31 and Liraglutide in Mouse Model of Obesity and MASLD

Original language description

Obesity and type 2 diabetes are key factors leading to metabolic dysfunction-associated steatotic liver disease (MASLD), characterized by fat accumulation in the liver. Anorexigenic peptides such as glucagon-like peptide 1 (GLP-1), its long-acting analogue liraglutide (LIRA), and prolactin-releasing peptide (PrRP) show promise for both obesity and MASLD treatment. Lipidization of peptides allows them to act centrally after peripheral administration, which have been shown in recent studies for palmitoylated PrRP31 (palm11-PrRP31). This study aims to investigate effects of palm11-PrRP31 and LIRA on a mouse model of obesity and MASLD. nMice with monosodium glutamate (MSG)-induced obesity, fed a high fat, high fructose and high cholesterol diet (FFC) or standard diet (STD) were used, while NMRI mice on either FFC or STD diet served as controls. MSG mice on FFC diet exhibited notable increases in body weight and liver weight, as well as elevated levels of leptin and CRP in plasma. Liver histology revealed a significant increase in the percentage of fat droplets and collagen in FFC diet mice, suggesting signs of fibrosis. After a period of seven months, a one-month intervention with palm11-PrRP31 and LIRA followed. Although body weight did not change significantly, there was a slight decrease in leptin level and a significant decrease in CRP level. Most importantly, a significant decrease in the percentage of fat droplets in the liver was observed after the treatment and the percentage of collagen in the liver was notably lower in the treated mice. nThe findings suggest that anorexigenic peptides may have potential for treating MASLD. However, further studies are required to determine the mechanism of action for its potential application. n

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů