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The histone chaperone Spt6 controls chromatin structure through its conserved N-terminal domain

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00639394" target="_blank" >RIV/61388963:_____/25:00639394 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11310/25:10505745

  • Result on the web

    <a href="https://doi.org/10.1016/j.molcel.2025.08.020" target="_blank" >https://doi.org/10.1016/j.molcel.2025.08.020</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.molcel.2025.08.020" target="_blank" >10.1016/j.molcel.2025.08.020</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The histone chaperone Spt6 controls chromatin structure through its conserved N-terminal domain

  • Original language description

    The disassembly and reassembly of nucleosomes by histone chaperones is an essential activity during eukaryotic transcription elongation. This highly conserved process maintains chromatin integrity by transiently removing nucleosomes as barriers and then restoring them in the wake of transcription. While transcription elongation requires multiple histone chaperones, there is little understanding of how most of them function and why so many are required. Here, we show that the histone chaperone Spt6 acts through its acidic, intrinsically disordered N-terminal domain (NTD) to bind histones and control chromatin structure. The Spt6 NTD is essential for viability, and its histone-binding activity is conserved between yeast and humans. The essential nature of the Spt6 NTD can be bypassed by changes in another histone chaperone, FACT, revealing a close functional connection between the two. Our results have led to a mechanistic model for dynamic cooperation between multiple histone chaperones during transcription elongation.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    <a href="/en/project/GX25-15442X" target="_blank" >GX25-15442X: Disentangling transient interactions in transcription elongation</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2025

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Molecular Cell

  • ISSN

    1097-2765

  • e-ISSN

    1097-4164

  • Volume of the periodical

    85

  • Issue of the periodical within the volume

    18

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    18

  • Pages from-to

    3407-3424

  • UT code for WoS article

    001576687800001

  • EID of the result in the Scopus database

    2-s2.0-105015644983