Mass spectrometry imaging of free-floating sections for the study of Alzheimer’s disease

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00639573" target="_blank" >RIV/61388963:_____/25:00639573 - isvavai.cz</a>

Result on the web

<a href="https://hdl.handle.net/11104/0369999" target="_blank" >https://hdl.handle.net/11104/0369999</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Mass spectrometry imaging of free-floating sections for the study of Alzheimer’s disease

Original language description

Sample collection and storage are important steps in the mass spectrometry imaging (MSI) sample preparation procedure. The recommended and most widely used procedure for MSI preparation involves freezing the sample in isopentane and storing it at -80 °C. On the other hand, the most common and general method for preserving biological samples in clinical practice is fixation in paraformaldehyde. In the present work, we investigated paraformaldehyde-fixed free-floating sections and studied their applicability for MSI analysis of lipids in the mouse brain. The developed procedure was used in a project aimed at determining lipid changes in double transgenic APP/PS1 mice, a model of Alzheimer’s-like pathology. Moreover, we examined the neuroprotective properties of palm11-PrRP31, an anorexigenic and glucose-lowering analog of prolactin-releasing peptide, using the MSI method. From the free-floating sections, we obtained lipid images without interferences or delocalization. In the APP/PS1 mouse model, we observed altered distribution of various lipids. An increased concentration of gangliosides (GM2 36:1, GM3 36:1) and phosphatidylinositols (PI 38:4, 36:4) and a decreased concentration of sulfatides (ST 18:0, 22:0, 24:0 and ST-OH 22:0, 24:0) were observed in areas where plaque accumulation occurred, compared to the surrounding tissue. In APP/PS1 mice treated with palm11-PrRP31 for 2 months, we found a reduced area of lipids associated with senile plaques. The results indicate that palm11-PrRP31 may potentially be useful in the treatment of neurodegenerative diseases. However, the mechanisms behind its neuroprotective function require further investigation.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/LX22NPO5104" target="_blank" >LX22NPO5104: National Institute for Research of Metabolic and Cardiovascular Diseases</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů