The farnesoid X receptor (FXR) antagonist 7β-isopropylchenodeoxycholic acid improves glucose metabolism in mice on a Western diet

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00639710" target="_blank" >RIV/61388963:_____/25:00639710 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/25:00639710

Result on the web

<a href="https://doi.org/10.1016/j.phrs.2025.107950" target="_blank" >https://doi.org/10.1016/j.phrs.2025.107950</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.phrs.2025.107950" target="_blank" >10.1016/j.phrs.2025.107950</a>

Result language

angličtina

Original language name

The farnesoid X receptor (FXR) antagonist 7β-isopropylchenodeoxycholic acid improves glucose metabolism in mice on a Western diet

Original language description

The significant roles of the farnesoid X receptor (FXR) and Takeda G protein-coupled receptor 5 (TGR5) in regulating metabolic pathways have recently been demonstrated. However, the precise effects of FXR inhibitors on glucose metabolism remain to be elucidated. In this study, we examined the impact of 7β-isopropylchenodeoxycholic acid (7β-ipCDCA), a dual FXR antagonist and TGR5 agonist, on impaired glucose metabolism in mice fed a Western diet. The dual FXR antagonistic/TGR5 agonistic activity of 7β-ipCDCA was confirmed through gene reporter assays. We evaluated its effects on glucose homeostasis using a glucose tolerance test in C57BL/6 mice fed a Western diet supplemented with sugar in drinking water for 24 weeks. The glucose-lowering mechanism was further investigated by measuring GLP-1 release, mRNA expression of glucose transporters, and relevant genes involved in glucose metabolism in intestinal, hepatic, white adipose, and kidney tissues, and in human NCI-H716 and murine GLUTag L cell lines. Additionally, bile acid metabolome and hepatic lipidome analyses were conducted. Results showed that 7β-ipCDCA improves glucose homeostasis altered by a Western diet in mice via enhanced GLP-1 secretion and decreased expression of glucose transporters in the ileum and kidneys. While its impact on liver function was marginal, 7β-ipCDCA increased plasma taurocholic acid levels. Furthermore, 7β-ipCDCA elevated hepatic triacylglycerols in mice on a chow diet, whereas mice on the Western diet were protected from triacylglycerol accumulation. This study highlights the role of FXR in regulating intestinal glucose transporters and GLP-1 secretion, emphasizing the therapeutic potential of combined FXR antagonists/TGR5 agonists in managing hyperglycemia.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30104 - Pharmacology and pharmacy

Project

<a href="/en/project/EH22_008%2F0004607" target="_blank" >EH22_008/0004607: New Technologies for Translational Research in Pharmaceutical Sciences /NETPHARM</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Pharmacological Research

ISSN

1043-6618

e-ISSN

1096-1186

Volume of the periodical

221

Issue of the periodical within the volume

November

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

13

Pages from-to

107950

UT code for WoS article

001576953700002

EID of the result in the Scopus database

2-s2.0-105016234769