Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F06%3A00043242" target="_blank" >RIV/61388971:_____/06:00043242 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast

Original language description

We found that mutating the RNP1 motif in the predicted RRM domain in yeast eukaryotic initiation factor 3 (eIF3) subunit b/PRT1 (prt1-rnp1) impairs its direct interactions in vitro with both eIF3a/TIF32 and eIF3j/HCR1. The rnp1 mutation in PRT1 confers temperature-sensitive translation initiation in vivo and reduces 40S-binding of eIF3 to native preinitiation complexes. Several findings indicate that the rnp1 lesion decreases recruitment of eIF3 to the 40S subunit by HCR1: (i) rnp1 strongly impairs theassociation of HCR1 with PRT1 without substantially disrupting the eIF3 complex; (ii) rnp1 impairs the 40S binding of eIF3 more so than the 40S binding of HCR1; (iii) overexpressing HCR1-R215I decreases the Ts_ phenotype and increases 40S-bound eIF3 in rnp1 cells; (iv) the rnp1 Ts_ phenotype is exacerbated by tif32-_6, which eliminates a binding determinant for HCR1 in TIF32; and (v) hcr1_ impairs 40S binding of eIF3 in otherwise wild-type cells

Czech name

Interakce RNP1 motivu v PRT1 s HCR1 usnadňuje vazbu eIF3 na 40S ribozóm v kvasinkách

Czech description

Práce pojednáva o studiu inciace translace a specifické úloze translačního iniciačního faktoru eIF3

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular and Cellular Biology

ISSN

0270-7306

e-ISSN

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Volume of the periodical

26

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

15

Pages from-to

2984-2998

UT code for WoS article

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EID of the result in the Scopus database

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