Phosphorylation of Type IA restriction-modification complex enzyme EcoKI on the HsdR subunit

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F07%3A00084812" target="_blank" >RIV/61388971:_____/07:00084812 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Phosphorylation of Type IA restriction-modification complex enzyme EcoKI on the HsdR subunit

Original language description

Phosphorylation of Type I restriction-modification enzymes EcoKI, EcoAI, and EcoR124I ? representatives of IA, IB, and IC families, respectively ? was analyzed in vivo by immunoblotting of endogenous phosphoproteins isolated from Escherichia coli strainsharbouring the corresponding hsd genes, and in vitro by phosphorylation assay using protein kinase present in subcellular fractions of E. coli. From all three restriction-modification enzymes, the HsdR subunit of EcoKI system was the only subunit, whichwas phosphorylated. Further, we present evidence that HsdR is phosphorylated in vivo only when co-produced with HsdM and HsdS subunits as part of assembled EcoKI restriction endonuclease, while the individually produced HsdR subunit is not phosphorylated. In vitro phosphorylation of the HsdR subunit of purified EcoKI endonuclease occurs on Thr, and is strictly dependent on the addition of a catalytic amount of cytoplasmic fraction isolated from E. coli

Czech name

Fosforylace podjednotky HsdR restrikčně-modifikačního enzymu Typu IA EcoKI

Czech description

Fosforylace restrikčně-modifikačních enzymů EcoKI, EcoAI a EcoR124I, zástupců skupin IA, IB a IC byla analyzovaná in vivo pomocí imunodetekce fosfoproteinů připravených z E. coli nesoucí odpovídající geny hsd a in vitro využitím protein kinázy přítomné vsubcelulárních frakcí E. coli. Ze všech testovaných enzymů byla fosforylace prokázána na podjednotce HsdR ezymu EcoKI a to pouze za podmínek, kdy je v buňce exprimována s podjednotkami HsdS a HsdM jako součást komplexní endonukleázy. Samostatně exprimovaná podjednotka HsdR fosorylována není. In vitro bylo prokázáno, že k fosforylaci dochází na treoninu cytoplazmatickou protein kinázou

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEMS Microbiology Letters

ISSN

0378-1097

e-ISSN

—

Volume of the periodical

270

Issue of the periodical within the volume

-

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

171-177

UT code for WoS article

—

EID of the result in the Scopus database

—