Activity of ribosomes and tmRNA of Streptomyces aureofaciens during development and stress conditions induced by changes in temperature and the presence of antibiotics
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F07%3A00095726" target="_blank" >RIV/61388971:_____/07:00095726 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Activity of ribosomes and tmRNA of Streptomyces aureofaciens during development and stress conditions induced by changes in temperature and the presence of antibiotics
Original language description
Streptomycetes are soil microorganisms exposed to various stresses that activate specialized responses whose coordinated action promotes growth under adverse conditions. Ribosomes having a highly cooperative structure are potential target for control mechanisms that generate signal and activate adaptive regulons or developmental programs. We examined how Streptomyces aureofaciens producing tetracycline responds to the presence of antibiotics and stresses induced by the changes in temperature. Tetracycline interacts with 16S RNA and decreases its thermodynamic stability. The drug also inhibits binding of ternary complex Phe-tRNA.EF-Tu.GTP to purified ribosomes. We have found that antibiotics that cause ribosome stall or pause could increase the requirement for tmRNA in the process trans-translation. Increase in tmRNA level was also demonstrated upon downshift in temperature
Czech name
Aktivita ribosomů a tmRNA Streptomyces aureofaciens v průběhu vývoje a za stresových podmínek indukovaných změnami teploty a přítomností antibiotik
Czech description
Streptomycety jsou půdní mikroorganismy vystavené různým stresům, které aktivují specializované odpovědi, umožňující růst v nepříznivých podmínkách. Ribosomy mají vysoce kooperativní strukturu a potenciál pro kontrolní mechanismy, které generují signál aaktivují adaptivní regulony nebo vývojové programy. Sledovali jsme jak Streptomyces aureofaciens produkující tetracyklin odpovídá na přítomnost antibiotik a na stresy indukované změnami teploty. Tetracyklin interaguje s 16S RNA a snižuje její termodynamickou stabilitu, inhibuje vazbu ternárního komplexu Phe-tRNA.EFTu.GTP na purifikované ribosomy a stimuluje syntézu tmRNA
Classification
Type
C - Chapter in a specialist book
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA203%2F05%2F0106" target="_blank" >GA203/05/0106: The role of trans-translation in cellular differentiation and resistance to produced antibiotics in Streptomycetes</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Communicating Current Research and Educational Topics
ISBN
978-84-611-9422-3
Number of pages of the result
7
Pages from-to
129-135
Number of pages of the book
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Publisher name
Formatex
Place of publication
Sevilla
UT code for WoS chapter
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