Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F07%3A00097671" target="_blank" >RIV/61388971:_____/07:00097671 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes
Original language description
The white rot fungus Irpex lacteus is able to decolorize such synthetic dyes as Reactive Orange 16 and Remazol Brilliant Blue R. Here,we demonstrate that this type of dye decolorization is mainly related to a laccase-like enzyme activity associated withfungal mycelium. In its bound form, the enzyme detected showed a pH optimum of 3.0 for the oxidation of ABTS, DMP and guaiacol, and a pH of 7.0 for syringaldazine. The highest enzymatic activity was obtained with ABTS as substrate. Enzyme activity was fully inhibited with 50 mM NaN3. Depending on the chemical structure of dyes, redox mediators had a positive effect on the dye decolorization by fungal mycelium.Enzyme isolated from fungal mycelium was able to decolorize synthetic dyes in vitro
Czech name
Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes
Czech description
Práce dokazuje, že proces dekolorizace syntetických barviv Reactive Orange 16 a Remazol Brilliant Blue R houbou Irpex lacteus je spojen oxidativním enzymem podobným lakasam a v tomto případě asociovaným s houbovým myceliem. Ve své vázané formě enzym vykazoval optimum pro oxidace ABTS, DMP a guaiacolu při pH 3.0 a při pH 7,0 pro oxidaci syringaldazinu. Nejvyšší enzymová aktivita byla při tom naměřena s ABTS. Aktivita byla inhibována 50 mM NaN3. Přidání redox mediátorů mělo pozitivní efekt na dekolorizacibarviv pomocí houbového mycelia. Enzym uvolněný z mycelia byl také schopen odbarvovat vybraná barviva za in vitro podmínek
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/IAA6020411" target="_blank" >IAA6020411: Mechanisms of biodegradation of environemntal organopollutants by ligninolytic fungi</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>O - Projekt operacniho programu<br>R - Projekt Ramcoveho programu EK
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bioresource Technology
ISSN
0960-8524
e-ISSN
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Volume of the periodical
99
Issue of the periodical within the volume
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Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
463-471
UT code for WoS article
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EID of the result in the Scopus database
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