Sequence analysis and heterologous expression of the lincomycin biosynthetic cluster of the type strain Streptomyces lincolnensis ATCC 25466
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F08%3A00315795" target="_blank" >RIV/61388971:_____/08:00315795 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Sequence analysis and heterologous expression of the lincomycin biosynthetic cluster of the type strain Streptomyces lincolnensis ATCC 25466
Original language description
A cosmid bearing an insert of 38217 bp covering the gene cluster and its flanking regions type strain Streptomyces lincolnensis ATCC 25466 was sequenced. Two relatively extensive sequence changes and several hundred point mutations were identified if compared with the previously published sequence of the lincomycin industrial strain Streptomyces lincolnensis 78-11. Analysis of the cluster flanking regions revealed its localization within the genome of S. lincolnensis ATCC 25466. The cluster-bearing cosmid was integrated into the chromosome of lincomycin non-producing strains Streptomyces coelicolor CH 999 and Streptomyces coelicolor M 145. The modified strains heterologously produced lincomycin, but the level dropped to approximately 1-3% of the production in S. lincolnensis ATCC 25466
Czech name
Analýza sekvence a heterologní exprese shluku pro biosyntézu linkomycinu z typového kmene Streptomyces lincolnensis ATCC 25466
Czech description
Byl sekvenován kosmid nesoucí shluk genů o velikosti 38217 pb, který byl izolován z typového kmene Streptomyces lincolnensis ATCC 25466. Porovnáním s již publikovanou sekvencí linkomycinového shluku z průmyslového kmene Streptomyces lincolnensis 78-11 byly zjištěny dva významné sekvenční rozdíly a dalších několik set bodových mutací. Kosmid nesoucí genový shluk byl vložen do chromozómů kmenů Streptomyces coelicolor CH 999 a Streptomyces coelicolor M 145, které linkomycin přirozeně neprodukují. Takto upravené kmeny produkovaly linkomycin s 1-3% výtěžnosti ve srovnání s typovým kmenem S. lincolnensis ATCC 25466
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Folia Microbiologica
ISSN
0015-5632
e-ISSN
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Volume of the periodical
53
Issue of the periodical within the volume
5
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
7
Pages from-to
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UT code for WoS article
000261763200002
EID of the result in the Scopus database
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