Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F11%3A00360791" target="_blank" >RIV/61388971:_____/11:00360791 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00792-011-0373-4" target="_blank" >http://dx.doi.org/10.1007/s00792-011-0373-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00792-011-0373-4" target="_blank" >10.1007/s00792-011-0373-4</a>

Result language

angličtina

Original language name

Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber

Original language description

Two thermophilic strains belonging to Geobacillus stearothermophilus and Meiothermus ruber, which naturally do not synthesize omega-alicyclic fatty acids (omega-FAs) were cultivated with cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl carboxylic acids. Gas chromatography-mass spectrometry analysis of fatty acid methyl and picolinyl esters showed that both strains are able to synthesize omega-FAs when cultivated with the appropriate precursor. The incorporation of cyclic acids influenced the whole FAcomposition as well as membrane fluidity. Membrane fluidity of intact cells was studied by measuring the fluorescence polarisation of the probe l,6-diphenyl-1,3,5-hexatriene incorporated into membrane lipid bilayers. Cytoplasmic membrane became more fluid with increasing content of omega-FAs. This is caused by considerable changes in lipid packing within the membrane induced by the presence of omega-FAs not found in the natural environment of Geobacillus and Meiothermus strains

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Extremophiles

ISSN

1431-0651

e-ISSN

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Volume of the periodical

15

Issue of the periodical within the volume

3

Country of publishing house

JP - JAPAN

Number of pages

7

Pages from-to

423-429

UT code for WoS article

000290037700012

EID of the result in the Scopus database

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