Structural analysis of mycolic acids from phenol-degrading strain of Rhodococcus erythropolis by liquid chromatography-tandem mass spectrometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F12%3A00387053" target="_blank" >RIV/61388971:_____/12:00387053 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22340/12:43893286 RIV/60461373:22330/12:43893286

Result on the web

<a href="http://dx.doi.org/10.1007/s12223-012-0156-z" target="_blank" >http://dx.doi.org/10.1007/s12223-012-0156-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-012-0156-z" target="_blank" >10.1007/s12223-012-0156-z</a>

Result language

angličtina

Original language name

Structural analysis of mycolic acids from phenol-degrading strain of Rhodococcus erythropolis by liquid chromatography-tandem mass spectrometry

Original language description

We used reversed phase liquid chromatography-electrospray ionization tandem mass spectrometry for direct analysis of mycolic acids (MAs) from four different cultivations of Rhodococcus erythropolis. This technique enabled us to identify and quantify thespecific molecular species of MAs directly from lipid extracts of the bacterium, including the determination of their basic characteristics such as retention time and mass spectra. We identified a total of 60 molecular species of MAs by means of LC/MS. In collision-induced dissociation tandem mass spectrometry, the [M-H](-) ions eliminated two residues, i.e., meroaldehyde and carboxylate anions containing alpha-alkyl chains. The structural information from these fragment ions affords structural assignment of the mycolic acids, including the lengths and number of double bond(s). Two strains, i.e., R. erythropolis CCM 2595 and genetically modified strain CCM 2595 pSRK 21 phe were cultivated on two different substrates (phenol and phenol w

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

6

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

473-483

UT code for WoS article

000310087200001

EID of the result in the Scopus database

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