Beta-tubulin paralogue tubC is frequently misidentified as the benA gene in Aspergillus section Nigri taxonomy: primer specificity testing ad taxonomic consequences

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F12%3A00389635" target="_blank" >RIV/61388971:_____/12:00389635 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3767/003158512X658123" target="_blank" >http://dx.doi.org/10.3767/003158512X658123</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3767/003158512X658123" target="_blank" >10.3767/003158512X658123</a>

Result language

angličtina

Original language name

Beta-tubulin paralogue tubC is frequently misidentified as the benA gene in Aspergillus section Nigri taxonomy: primer specificity testing ad taxonomic consequences

Original language description

beta-tubulin (benA, tub-2) and calmodulin (caM) are crucial genes in the taxonomy of Aspergillus section Nigri. Widely used beta-tubulin primers are not specific for the benA gene for some taxa and preferentially amplify the tubC paralogue. Sequences ofthe tubC paralogue are widely combined with benA sequences in recent taxonomical works as well as other works, resulting in incongruent trees. In this study we newly provide benA sequences for several ex-type strains, which were characterised using the tubC gene only. We designed a highly specific forward primer to benA designated Ben2f for use in Aspergillus section Nigri, and tested specificity of numerous primer combinations to beta-tubulin paralogs. The primer pairs with the highest specificity to the benA gene and functional across species in section Nigri includes Ben2f/Bt2b, Ben2f/T22 and T10/T22. We also provide tools based on codon usage bias analysis that reliably distinguish both paralogues. Exon/intron arrangement is the nex

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Persoonia

ISSN

0031-5850

e-ISSN

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Volume of the periodical

29

Issue of the periodical within the volume

DEC 2012

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

1-10

UT code for WoS article

000313744900001

EID of the result in the Scopus database

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