Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00376216" target="_blank" >RIV/61388971:_____/13:00376216 - isvavai.cz</a>
Alternative codes found
RIV/67985807:_____/13:00376216 RIV/60076658:12520/13:43885239 RIV/61388971:_____/13:00425117
Result on the web
<a href="http://dx.doi.org/10.1016/j.mcm.2011.12.029" target="_blank" >http://dx.doi.org/10.1016/j.mcm.2011.12.029</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.mcm.2011.12.029" target="_blank" >10.1016/j.mcm.2011.12.029</a>

Result language
angličtina
Original language name
Estimation of diffusivity of phycobilisomes on thylakoid membrane based on spatio-temporal FRAP images
Original language description
The determination of phycobilisome diffusivity (diffusion coefficient D) on thylakoid membrane from fluorescence recovery after photobleaching (FRAP) experiments is usually done by analytical models. However, analytical models need some unrealistic conditions to be supposed. This study describes the development of a method based on finite difference approximation of the process governed by the Fickian diffusion equation and on the minimization of an objective function representing the disparity betweenthe experimental and simulated time-varying concentration profiles. Our method improves on other models by accounting for experimentally measured time-varying Dirichlet boundary conditions, and can include a reaction term as well. As a result we obtain both the overall (time averaged) diffusion coefficient D and the sequence of diffusivities Dj based on two successive fluorescence profiles in j-th time interval. Due to the noisy data, we cope with an inverse ill-posed problem and a regul
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BA - General mathematics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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