The C-terminal domain of Brd2 is important for chromatin interaction and regulation of transcription and alternative splicing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00424234" target="_blank" >RIV/61388971:_____/13:00424234 - isvavai.cz</a>

Alternative codes found

RIV/61388955:_____/13:00422977 RIV/68378050:_____/13:00422977

Result on the web

<a href="http://dx.doi.org/10.1091/mbc.E13-06-0303" target="_blank" >http://dx.doi.org/10.1091/mbc.E13-06-0303</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1091/mbc.E13-06-0303" target="_blank" >10.1091/mbc.E13-06-0303</a>

Result language

angličtina

Original language name

The C-terminal domain of Brd2 is important for chromatin interaction and regulation of transcription and alternative splicing

Original language description

Brd2 is a member of the bromodomain extra terminal (BET) protein family, which consists of four chromatin-interacting proteins that regulate gene expression. Each BET protein contains two N-terminal bromodomains, which recognize acetylated histones, andthe C-terminal protein-protein interaction domain. Using a genome-wide screen, we identify 1450 genes whose transcription is regulated by Brd2. In addition, almost 290 genes change their alternative splicing pattern upon Brd2 depletion. Brd2 is specifically localized at promoters of target genes, and our data show that Brd2 interaction with chromatin cannot be explained solely by histone acetylation. Using coimmunoprecipitation and live-cell imaging, we show that the C-terminal part is crucial for Brd2association with chromatin. Live-cell microscopy also allows us to map the average binding time of Brd2 to chromatin and quantify the contributions of individual Brd2 domains to the interaction with chromatin. Finally, we show that bromod

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Biology of the Cell

ISSN

1059-1524

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

22

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

3557-3568

UT code for WoS article

000328124600007

EID of the result in the Scopus database

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