Structural integrity of the PCI domain of eIF3a/TIF32 is required for mRNA recruitment to the 43S pre-initiation complexes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00428353" target="_blank" >RIV/61388971:_____/14:00428353 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gkt1369" target="_blank" >http://dx.doi.org/10.1093/nar/gkt1369</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkt1369" target="_blank" >10.1093/nar/gkt1369</a>

Result language

angličtina

Original language name

Structural integrity of the PCI domain of eIF3a/TIF32 is required for mRNA recruitment to the 43S pre-initiation complexes

Original language description

Transfer of genetic information from genes into proteins is mediated by messenger RNA (mRNA) that must be first recruited to ribosomal pre-initiation complexes (PICs) by a mechanism that is still poorly understood. Recent studies showed that besides eIF4F and poly(A)-binding protein, eIF3 also plays a critical role in this process, yet the molecular mechanism of its action is unknown. We showed previously that the PCI domain of the eIF3c/NIP1 subunit of yeast eIF3 is involved in RNA binding. To assess the role of the second PCI domain of eIF3 present in eIF3a/TIF32, we performed its mutational analysis and identified a 10-Ala-substitution (Box37) that severely reduces amounts of model mRNA in the 43-48S PICs in vivo as the major, if not the only, detectable defect. Crystal structure analysis of the a/TIF32-PCI domain at 2.65-A resolution showed that it is required for integrity of the eIF3 core and, similarly to the c/NIP1-PCI, is capable of RNA binding. The putative RNA-binding surfac

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

<a href="/en/project/GAP305%2F10%2F0335" target="_blank" >GAP305/10/0335: Deciphering molecular details of the stringent decoding of the AUG start codon during eukaryotic translation initiation.</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

—

Volume of the periodical

42

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

17

Pages from-to

4123-4139

UT code for WoS article

000334758600061

EID of the result in the Scopus database

—