Intracellular sequestration of zinc, cadmium and silver in Hebeloma mesophaeum and characterization of its metallothionein genes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00429961" target="_blank" >RIV/61388971:_____/14:00429961 - isvavai.cz</a>
Alternative codes found
RIV/61389005:_____/14:00429961 RIV/60461373:22330/14:43897719
Result on the web
<a href="http://dx.doi.org/10.1016/j.fgb.2014.03.003" target="_blank" >http://dx.doi.org/10.1016/j.fgb.2014.03.003</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fgb.2014.03.003" target="_blank" >10.1016/j.fgb.2014.03.003</a>

Result language
angličtina
Original language name
Intracellular sequestration of zinc, cadmium and silver in Hebeloma mesophaeum and characterization of its metallothionein genes
Original language description
Sequestration of intracellular heavy metals in eukaryotes involves compartmentalization and binding with cytosolic, cysteine-rich metallothionein (MT) peptides. We examined the roles of these processes in handling of zinc (Zn), cadmium (Cd) and silver (Ag) in sporocarps and a metal-exposed extraradical mycelium of Hebeloma mesophaeum, the Zn-accumulating ectomycorrhizal (EM) species frequently associated with metal disturbed sites. Size exclusion chromatography revealed that the majority of Zn and Cd inthe sporocarps and mycelium was contained in a low molecular mass fraction attributable to compartmentalized metal. The staining of hyphal cells with the Zn-specific Zinquin and Cd-specific Leadmium fluorescent tracers labeled Zn and Cd in small, punctuated vesicles and vacuoles, respectively. By contrast, the sporocarp and mycelium Ag was associated with cysteine-rich, 5-kDa peptides. The peptides of the same size were also identified in minor Zn and Cd complexes from the metal-exposed
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
<a href="/en/project/GAP504%2F11%2F0484" target="_blank" >GAP504/11/0484: Hyperaccumulation of heavy metals in macrofungi - molecular, geomycological and ecotaxonomic aspects</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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