Preservation of basidiomycete strains on perlite using different protocols

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F14%3A00435964" target="_blank" >RIV/61388971:_____/14:00435964 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.myc.2014.01.006" target="_blank" >http://dx.doi.org/10.1016/j.myc.2014.01.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.myc.2014.01.006" target="_blank" >10.1016/j.myc.2014.01.006</a>

Result language

angličtina

Original language name

Preservation of basidiomycete strains on perlite using different protocols

Original language description

For preservation of 31 basidiomycete strains on perlite in cryovials we used five different perlite protocols to compare their applicability in laboratories with different equipment, namely a viability of the controlled freezing device or the electric deep-freezer and liquid nitrogen supply. The viability of the strains, macromorphological characteristics and the production of laccase were tested after 48 h, six months and one year of storage in the respective device. Our results indicated that the different response to the freezing/thawing process is an intrinsic feature of the respective strain. Nevertheless, the highest viability and preservation of laccase production in our tested strains was found when we used prefreezing to -80 degrees Cat a freezing rate of 1 degrees C/min in a programmable IceCube 1800 freezer or in freezing container Mr. Frosty before storage in liquid nitrogen or at ultra-low temperature freezer at -80 degrees C, respectively. The two abovementioned protocols

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GAP504%2F12%2F0709" target="_blank" >GAP504/12/0709: Basidiomycetous fungi: exploration and preservation of biodiversity and involvement in environmental decomposition processes</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mycoscience

ISSN

1340-3540

e-ISSN

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Volume of the periodical

55

Issue of the periodical within the volume

6

Country of publishing house

JP - JAPAN

Number of pages

10

Pages from-to

439-448

UT code for WoS article

000342076300004

EID of the result in the Scopus database

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