Concomitant use of polarization and positive phase contrast microscopy for the study of microbial cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F15%3A00464007" target="_blank" >RIV/61388971:_____/15:00464007 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s12223-015-0397-8" target="_blank" >http://dx.doi.org/10.1007/s12223-015-0397-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-015-0397-8" target="_blank" >10.1007/s12223-015-0397-8</a>

Result language

angličtina

Original language name

Concomitant use of polarization and positive phase contrast microscopy for the study of microbial cells

Original language description

Polarization and positive phase contrast microscope were concomitantly used in the study of the internal structure of microbial cells. Positive phase contrast allowed us to view even the fine cell structure with a refractive index approaching that of the surrounding environment, e.g., the cytoplasm, and transferred the invisible phase image to a visible amplitude image. With polarization microscopy, crossed polarizing filters together with compensators and a rotary stage showed the birefringence of different cell structures. Material containing algae was collected in ponds in SA1/2koA (TM) ice and Zbeno villages (KA (TM) ivoklat region). The objects were studied in laboratory microscopes LOMO MIN-8 Sankt Petersburg and Polmi A Carl Zeiss Jena fitted with special optics for positive phase contrast, polarizers, analyzers, compensators, rotary stages, and digital SLR camera Nikon D 70 for image capture. Anisotropic granules were found in the cells of flagellates of the order Euglenales, in green algae of the orders Chlorococcales and Chlorellales, and in desmid algae of the order Desmidiales.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

—

Volume of the periodical

60

Issue of the periodical within the volume

6

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

545-550

UT code for WoS article

000362589400011

EID of the result in the Scopus database

2-s2.0-84943354992