Crystallization of nepenthesin I using a low-pH crystallization screen

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00457610" target="_blank" >RIV/61388971:_____/16:00457610 - isvavai.cz</a>

Alternative codes found

RIV/61389013:_____/16:00457610 RIV/00216208:11310/16:10324222 RIV/86652036:_____/16:00508211

Result on the web

<a href="http://dx.doi.org/10.1107/S2053230X15022323" target="_blank" >http://dx.doi.org/10.1107/S2053230X15022323</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1107/S2053230X15022323" target="_blank" >10.1107/S2053230X15022323</a>

Result language

angličtina

Original language name

Crystallization of nepenthesin I using a low-pH crystallization screen

Original language description

Nepenthesins are aspartic proteases secreted by carnivorous pitcher plants of the genus Nepenthes. They significantly differ in sequence from other plant aspartic proteases. This difference, which provides more cysteine residues in the structure of nepenthesins, may contribute to their unique stability profile. Recombinantly produced nepenthesin 1 (rNep1) from N. gracilis in complex with pepstatin A was crystallized under two different crystallization conditions using a newly formulated low-pH crystallization screen. The diffraction data were processed to 2.9 and 2.8 Å resolution, respectively. The crystals belonged to space group P212121, with unit-cell parameters a = 86.63, b = 95.90, c = 105.40 Å, [alpha] = [beta] = [gamma] = 90° and a = 86.28, b = 97.22, c = 103.78 Å, [alpha] = [beta] = [gamma] = 90°, respectively. Matthews coefficient and solvent-content calculations suggest the presence of two molecules of rNep1 in the asymmetric unit. Here, the details of the crystallization experiment and analysis of the X-ray data are reported.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS

ISSN

2053-230X

e-ISSN

—

Volume of the periodical

72

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

5

Pages from-to

24-28

UT code for WoS article

000369379700005

EID of the result in the Scopus database

2-s2.0-84954317484