In-depth analysis of cis-determinants that either promote or inhibit reinitiation on GCN4 mRNA after translation of its four short uORFs
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00459969" target="_blank" >RIV/61388971:_____/16:00459969 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1261/rna.055046.115" target="_blank" >http://dx.doi.org/10.1261/rna.055046.115</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1261/rna.055046.115" target="_blank" >10.1261/rna.055046.115</a>
Alternative languages
Result language
angličtina
Original language name
In-depth analysis of cis-determinants that either promote or inhibit reinitiation on GCN4 mRNA after translation of its four short uORFs
Original language description
Translational control in eukaryotes is exerted by many means, one of which involves a ribosome translating multiple cistrons per mRNA as in bacteria. It is called reinitiation (REI) and occurs on mRNAs where the main ORF is preceded by a short upstream uORF(s). Some uORFs support efficient REI on downstream cistrons, whereas some others do not. The mRNA of yeast transcriptional activator GCN4 contains four uORFs of both types that together compose an intriguing regulatory mechanism of its expression responding to nutrients' availability and various stresses. Here we subjected all GCN4 uORFs to a comprehensive analysis to identify all REI-promoting and inhibiting cis-determinants that contribute either autonomously or in synergy to the overall efficiency of REI on GCN4. We found that the 3' sequences of uORFs 1-3 contain a conserved AU(1-2)A/UUAU(2) motif that promotes REI in position-specific, autonomous fashion such as the REI-promoting elements occurring in 5' sequences of uORF1 and uORF2.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GAP305%2F11%2F0172" target="_blank" >GAP305/11/0172: Molecular basis of the gene-specific translational control mechanism of yeast GCN4 and its mammalian functional homologue ATF4.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
R N A
ISSN
1355-8382
e-ISSN
—
Volume of the periodical
22
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
542-558
UT code for WoS article
000372766700006
EID of the result in the Scopus database
2-s2.0-84962550055