Formaldehyde fixation is detrimental to actin cables in glucose-depleted S. cerevisiae cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00462340" target="_blank" >RIV/61388971:_____/16:00462340 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.15698/mic2016.05.499" target="_blank" >http://dx.doi.org/10.15698/mic2016.05.499</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15698/mic2016.05.499" target="_blank" >10.15698/mic2016.05.499</a>

Result language
angličtina
Original language name
Formaldehyde fixation is detrimental to actin cables in glucose-depleted S. cerevisiae cells
Original language description
Actin filaments form cortical patches and emanating cables in fermenting cells of Saccharomyces cerevisiae. This pattern has been shown to be depolarized in glucose-depleted cells after formaldehyde fixation and staining with rhodamine-tagged phalloidin. Loss of actin cables in mother cells was remarkable. Here we extend our knowledge on actin in live glucosedepleted cells co-expressing the marker of actin patches (Abp1-RFP) with the marker of actin cables (Abp140-GFP). Glucose depletion resulted in appearance of actin patches also in mother cells. However, even after 80 min of glucose deprivation these cells showed a clear network of actin cables labeled with Abp140-GFP in contrast to previously published data.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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