In-silico driven engineering of enantioselectivity of a penicillin G acylase towards active pharmaceutical ingredients

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F16%3A00483930" target="_blank" >RIV/61388971:_____/16:00483930 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/16:10368393

Result on the web

<a href="http://dx.doi.org/10.1016/j.molcatb.2016.11.014" target="_blank" >http://dx.doi.org/10.1016/j.molcatb.2016.11.014</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molcatb.2016.11.014" target="_blank" >10.1016/j.molcatb.2016.11.014</a>

Result language

angličtina

Original language name

In-silico driven engineering of enantioselectivity of a penicillin G acylase towards active pharmaceutical ingredients

Original language description

Penicillin G acylase is one of the most employed enzymes in the pharmaceutical industry due to its role in the biotransformation of semi-synthetic Beta-lactam antibiotics. Recently, the enantioselectivity of the penicillin G acylase markedly broadened its application potential. In this study, we have evaluated effects of in-silico replacements of acyl-binding subsite residue Phe24Beta of the enzyme from Achromobacter sp. CCM 4824 to seven markedly smaller amino acids on its enantioselectivity towards industrially relevant compounds. Models of the two most promising mutants bearing substitutions PheBeta24Ala and PheBeta24Cys were investigated using molecular docking calculations. The Cys substitution revealed much better enantioselectivity traits with a set of seven substrates. To verify the relevance of in-silico predictions, we constructed a PGA. A + Phe24BetaCys mutant and determined its enantioselectivity in biocatalytic reactions. Since we experimentally confirmed all these predictions, we expanded our in-silico analysis to another set of seven compounds: the prediction suggested increased enantioselectivity for N-phenylacetyl-p-F-alfa-phenylalanine. The (R)-enantiomer of this substrate is used as a building block in synthesis of important anti-cancer agent Abarelix. The enantioselectivity of PGA. A + Phe24BetaCys mutant towards this substrate was improved by 75percent reaching E-value of about 70. Our results suggest the rapid identification of interesting replacements altering enantioselectivity using in-silico approach as the way for further expanding biotechnological application of penicillin G acylase

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Molecular Catalysis B-Enzymatic

ISSN

1381-1177

e-ISSN

—

Volume of the periodical

133

Issue of the periodical within the volume

Supplement 1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

53-59

UT code for WoS article

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EID of the result in the Scopus database

2-s2.0-85009773584