Efficient screening of potential cellulases and hemicellulases produced by Bosea sp FBZP-16 using the combination of enzyme assays and genome analysis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00473897" target="_blank" >RIV/61388971:_____/17:00473897 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s11274-016-2198-x" target="_blank" >http://dx.doi.org/10.1007/s11274-016-2198-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11274-016-2198-x" target="_blank" >10.1007/s11274-016-2198-x</a>

Result language

angličtina

Original language name

Efficient screening of potential cellulases and hemicellulases produced by Bosea sp FBZP-16 using the combination of enzyme assays and genome analysis

Original language description

dentification of bacteria that produce carbohydrolytic enzymes is extremely important given the increased demand for these enzymes in many industries. Twenty lignocellulose-degrading bacterial isolates from Algerian compost and different soils were screened for their potential to produce different enzymes involved in biomass deconstruction. Based on 16S rRNA gene sequencing, the isolates belonged to Proteobacteria and Actinobacteria. Differences among species were reflected both as the presence/absence of enzymes or at the level of enzyme activity. Among the most active species, Bosea sp. FBZP-16 demonstrated cellulolytic activity on both amorphous cellulose (CMC) and complex lignocellulose (wheat straw) and was selected for whole-genomic sequencing. The genome sequencing revealed the presence of a complex enzymatic machinery required for organic matter decomposition. Analysis of the enzyme-encoding genes indicated that multiple genes for endoglucanase, xylanase, beta-glucosidase and beta-mannosidase are present in the genome with enzyme activities displayed by the bacterium, while other enzymes, such as certain cellobiohydrolases, were not detected at the genomic level. This indicates that a combination of functional screening of bacterial cultures with the use of genome-derived information is important for the prediction of potential enzyme production. These results provide insight into their possible exploitation for the production of fuels and chemicals derived from plant biomass.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

World Journal of Microbiology and Biotechnology

ISSN

0959-3993

e-ISSN

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Volume of the periodical

33

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

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UT code for WoS article

000394047000009

EID of the result in the Scopus database

2-s2.0-85009105950