Seasonal variation and distribution of total and active microbial community of beta-glucosidase encoding genes in coniferous forest soil
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00474445" target="_blank" >RIV/61388971:_____/17:00474445 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.soilbio.2016.11.003" target="_blank" >http://dx.doi.org/10.1016/j.soilbio.2016.11.003</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.soilbio.2016.11.003" target="_blank" >10.1016/j.soilbio.2016.11.003</a>
Alternative languages
Result language
angličtina
Original language name
Seasonal variation and distribution of total and active microbial community of beta-glucosidase encoding genes in coniferous forest soil
Original language description
Cellulose is the most abundant polysaccharide in the dead plant biomass, and its degradation is an important part of global carbon cycle. Beta-Glucosidases complete the final step of cellulose hydrolysis by converting cellobiose to glucose. Genetic potential and expression of Beta-glucosidase genes were studied in the topsoil of a Picea abies forest in two contrasting seasons. These seasons were the summer, representing the peak of plant photosynthetic activity, and late winter, after an extended period with no photosynthate input. Fungal and bacterial beta-glucosidase genes belonging to glycoside hydrolase families GH1 and GH3 amplified from DNA and RNA and amplicon pools were analyzed. Transcript pool were largely corresponded to gene pools, although some abundant transcripts were not found in the gene pool. The major reservoirs of Beta-glucosidase genes were the fungal phylum Ascomycota and Basidiomycota and the bacterial phyla Firmicutes, Actinobacteria, Proteobacteria Acidobacteria and Deinococcus-Thermus. This indicates that a diverse microbial community utilized cellobiose. Seasonality influenced both genetic diversity of Beta-glucosidase genes and their expression. The results indicate that a complex community of bacteria and fungi expresses beta-glucosidases in forest soils. Even Beta-glucosidase genes showing low abundance in DNA may be functionally important as revealed by their high expression especially in basidiomycota. The functional diversity in the studied ecosystem clearly exhibited a seasonal pattern.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA16-08916S" target="_blank" >GA16-08916S: Drivers of fungal community composition and function in coniferous forest ecosystems</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Soil Biology and Biochemistry
ISSN
0038-0717
e-ISSN
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Volume of the periodical
105
Issue of the periodical within the volume
February
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
71-80
UT code for WoS article
000392788600010
EID of the result in the Scopus database
2-s2.0-84997293631