Glycan-decorated HPMA copolymers as high-affinity lectin ligands

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F17%3A00474869" target="_blank" >RIV/61388971:_____/17:00474869 - isvavai.cz</a>

Alternative codes found

RIV/61389013:_____/17:00474869 RIV/61388963:_____/17:00474869

Result on the web

<a href="http://dx.doi.org/10.1039/c7py00271h" target="_blank" >http://dx.doi.org/10.1039/c7py00271h</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c7py00271h" target="_blank" >10.1039/c7py00271h</a>

Result language

angličtina

Original language name

Glycan-decorated HPMA copolymers as high-affinity lectin ligands

Original language description

Novel conjugates of N-(2- hydroxypropyl) methacrylamide (HPMA) copolymers tethered with chitooligosaccharidic epitopes of varying lengths were shown to be potent ligands of a model lectin, wheat germ agglutinin (WGA). The azide-functionalized oligosaccharidic epitopes were prepared by the action of Tyr470Asn mutant alpha-N-acetylhexosaminidase from Talaromyces flavus in a single reaction step and were conjugated to HPMA copolymer precursors in a defined pattern and density through Cu+- catalyzedazide-alkyne cycloaddition. The soluble, biocompatible, and structurally flexible synthetic glycopolymers were studied for their binding to WGA in a competitive enzyme-linked lectin assay (ELLA), and the kinetics of interaction were analyzed by surface plasmon resonance (SPR). To the best of our knowledge, this study presents the first HPMA copolymers derivatized with long oligosaccharides that demonstrate high affinity to a lectin target. The binding affinities in the low nanomolar and subnanomolar ranges place the prepared glycopolymers among the best WGA ligands reported to date. This study demonstrates the targeting potential of these glycopolymers for therapeutically relevant lectins.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Polymer Chemistry

ISSN

1759-9954

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

17

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

2647-2658

UT code for WoS article

000400337000008

EID of the result in the Scopus database

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