sigma(I) from Bacillus subtilis: Impact on Gene Expression and Characterization of sigma(I)-Dependent Transcription That Requires New Types of Promoters with Extended-35 and-10 Elements

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00493283" target="_blank" >RIV/61388971:_____/18:00493283 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/18:10383396

Result on the web

<a href="http://dx.doi.org/10.1128/JB.00251-18" target="_blank" >http://dx.doi.org/10.1128/JB.00251-18</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/JB.00251-18" target="_blank" >10.1128/JB.00251-18</a>

Result language

angličtina

Original language name

sigma(I) from Bacillus subtilis: Impact on Gene Expression and Characterization of sigma(I)-Dependent Transcription That Requires New Types of Promoters with Extended-35 and-10 Elements

Original language description

The sigma(I) sigma factor from Bacillus subtilis is a a factor associated with RNA polymerase (RNAP) that was previously implicated in adaptation of the cell to elevated temperature. Here, we provide a comprehensive characterization of this transcriptional regulator. By transcriptome sequencing (RNA-seq) of wild-type (wt) and sigma(I)-null strains at 37 degrees C and 52 degrees C, we identified -130 genes affected by the absence of sigma(I). Further analysis revealed that the majority of these genes were affected indirectly by sigma(I). The sigma(I) regulon, i.e., the genes directly regulated by sigma(I), consists of 16 genes, of which eight (the dhb and yku operons) are involved in iron metabolism. The involvement of sigma(I) in iron metabolism was confirmed phenotypically. Next, we set up an in vitro transcription system and defined and experimentally validated the promoter sequence logo that, in addition to -35 and -10 regions, also contains extended -35 and -10 motifs. Thus, sigma(I)-dependent promoters are relatively information rich in comparison with most other promoters. In summary, this study supplies information about the least-explored sigma factor from the industrially important model organism B. subtilis. n

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Bacteriology

ISSN

0021-9193

e-ISSN

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Volume of the periodical

200

Issue of the periodical within the volume

17

Country of publishing house

US - UNITED STATES

Number of pages

15

Pages from-to

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UT code for WoS article

000441290900010

EID of the result in the Scopus database

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