Bordetella pertussis and Bordetella bronchiseptica filamentous hemagglutinins are processed at different sites
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00494800" target="_blank" >RIV/61388971:_____/18:00494800 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/18:10376037
Result on the web
<a href="http://dx.doi.org/10.1002/2211-5463.12474" target="_blank" >http://dx.doi.org/10.1002/2211-5463.12474</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/2211-5463.12474" target="_blank" >10.1002/2211-5463.12474</a>
Alternative languages
Result language
angličtina
Original language name
Bordetella pertussis and Bordetella bronchiseptica filamentous hemagglutinins are processed at different sites
Original language description
Filamentous hemagglutinin (FHA) mediates adherence and plays an important role in lower respiratory tract infections by pathogenic Bordetellae. The mature FHA proteins of B. pertussis (Bp-FHA) and the B. bronchiseptica (Bb-FHA) are generated by processing of the respective FhaB precursors by the autotransporter subtilisin-type protease SphB1. We have used bottom-up proteomics with differential O-16/O-18 labeling and show that despite high-sequence conservation of the corresponding FhaB segments, the mature Bp-FHA (similar to 230 kDa) and Bb-FHA (similar to 243 kDa) proteins are processed at different sites of FhaB, after the Ala-2348 and Lys-2479 residues, respectively. Moreover, protease surface accessibility probing by oncolumn (on-line) digestion of the Bp-FHA and Bb-FHA proteins yielded different peptide patterns, revealing structural differences in the N-terminal and C-terminal domains of the Bp-FHA and Bb-FHA proteins. These data indicate specific structural variations between the highly homologous FHA proteins.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEBS Open Bio
ISSN
2211-5463
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
1256-1266
UT code for WoS article
000445645300007
EID of the result in the Scopus database
2-s2.0-85050929839