In-Vitro Activity of Silybin and Related Flavonolignans against Leishmania infantum and L-donovani

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00495967" target="_blank" >RIV/61388971:_____/18:00495967 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3390/molecules23071560" target="_blank" >http://dx.doi.org/10.3390/molecules23071560</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/molecules23071560" target="_blank" >10.3390/molecules23071560</a>

Result language

angličtina

Original language name

In-Vitro Activity of Silybin and Related Flavonolignans against Leishmania infantum and L-donovani

Original language description

Flavonolignans from the seeds of the milk thistle (Silybum marianum) have been extensively used in folk medicine for centuries. Confirmation of their properties as hepatoprotective, antioxidant and anticancer has been obtained using standardized extracts and purified flavonolignans. Information on their potential effect on Leishmania is very scarce. We have investigated the effect of silymarin, silybin and related flavonolignans on the multiplication of promastigotes in vitro and ex vivo on intracellular amastigotes of L. infantum (Li) and L. donovani (Ld), causative agents of human and canine visceral leishmaniasis (VL). In addition, the potential synergistic effect of the most active molecule and well-established antileishmanial drugs against promastigotes was explored. Dehydroisosilybin A elicited the highest inhibition against Ld and Li promastigotes with an approximate IC50 of 90.23 mu M. This molecule showed a moderate synergism with amphotericin B (AmB) but not with Sb-III or paromomycin, although it was ineffective against amastigotes. Antileishmanial activity on intracellular amastigotes of the two diastereoisomers of dehydrosilybin (10 mu M) was comparable to that elicited by 0.1 mu M AmB. Antiproliferative activity and safety of flavonolignans suggest the interest of exploring their potential value in combination therapy against VL.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30310 - Parasitology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecules

ISSN

1420-3049

e-ISSN

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Volume of the periodical

23

Issue of the periodical within the volume

7

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

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UT code for WoS article

000445301800063

EID of the result in the Scopus database

2-s2.0-85049443549