Nanodiamonds as Effective Vectors for Micro RNA-Based Drug Delivery

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00499815" target="_blank" >RIV/61388971:_____/18:00499815 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Nanodiamonds as Effective Vectors for Micro RNA-Based Drug Delivery
Original language description
Micro RNAs are short non-coding RNA molecules, which are important regulators of cell functions. Deregulation of certain micro RNA level is contributing factor to tumor formation and progression. Possible gene therapy by modulation of the micro RNA level is currently considered. Recently it has been proved that nano diamonds coated with PEI serve as efficient transfection and monitoring system. The aim of our work was to deliver micro RNA inhibitor and micro RNA mimic sequences into specialized mammalianncells using fluorescent nano diamond particles and to test the nano complex uptake and cell compatibility during primary in vivo application. Short RNA molecules were bound to the PEI coated nano diamonds, transported into the colon cancer cell line in vitro and observed in a confocal microscope. The miRNA mimic coated nano diamonds were used for complex uptake and toxicity study in peritoneal macrophages and splenocytes after ex vivo stimulation or in vivo administration. Due to the possibility of continuousnmonitoring of carrier internalization, its transfection efficacy, and cell compatibility, the fluorescent nano diamonds represent a suitable RNA carrier with a potential use in gene therapy.
Czech name
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Czech description
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Project
<a href="/en/project/NV15-33094A" target="_blank" >NV15-33094A: Nanofiber drug carriers for controlled release of wound healing substances based on the encapsulation of functionalized nanodiamond particles</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical
Drug Designing & Intellectual Properties International Journal
ISSN
2637-4706
e-ISSN
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Volume of the periodical
1
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
112-117
UT code for WoS article
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EID of the result in the Scopus database
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