6,7-dimethoxy-coumarin as a probe of hydration dynamics in biologically relevant systems

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F18%3A00506449" target="_blank" >RIV/61388971:_____/18:00506449 - isvavai.cz</a>

Alternative codes found

RIV/61388955:_____/18:00493474

Result on the web

<a href="https://iopscience.iop.org/article/10.1088/2050-6120/aaaa05" target="_blank" >https://iopscience.iop.org/article/10.1088/2050-6120/aaaa05</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/2050-6120/aaaa05" target="_blank" >10.1088/2050-6120/aaaa05</a>

Result language

angličtina

Original language name

6,7-dimethoxy-coumarin as a probe of hydration dynamics in biologically relevant systems

Original language description

Coumarin derivatives are well known fluorescence reporters for investigating biological systems due to their strong micro-environment sensitivity. Despite having wide range of environment sensitive fluorescence probes, the potential of 6,7-dimethoxy-coumarin has not been studied extensively so far. With a perspective of its use in protein studies, namely using the unnatural amino acid technology or as a substrate for hydrolase enzymes, we study acetyloxymethyl-6,7-dimethoxycoumarin (Ac-DMC). We investigate the photophysics and hydration dynamics of this dye in aerosol-OT (AOT) reverse micelles at various water contents using the time dependent fluorescence shift (TDFS) method. The TDFS response in AOT reverse micelles from water/surfactant ratio of 0 to 20 confirms its sensitivity towards the hydration and mobility of its microenvironment. Moreover, we show that the fluorophore can be efficiently quenched by halide ions. Hence, we conclude that the 6,7-dimethoxy-methylcoumarin fluorophore is useful for studying hydration parameters in biologically relevant systems.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Methods and Applications in Fluorescence

ISSN

2050-6120

e-ISSN

—

Volume of the periodical

6

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

025005

UT code for WoS article

000425031200001

EID of the result in the Scopus database

2-s2.0-85048126917