Response of the Green Alga Chlamydomonas reinhardtii to the DNA Damaging Agent Zeocin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00508305" target="_blank" >RIV/61388971:_____/19:00508305 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2073-4409/8/7/735" target="_blank" >https://www.mdpi.com/2073-4409/8/7/735</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/cells8070735" target="_blank" >10.3390/cells8070735</a>
Alternative languages
Result language
angličtina
Original language name
Response of the Green Alga Chlamydomonas reinhardtii to the DNA Damaging Agent Zeocin
Original language description
DNA damage is a ubiquitous threat endangering DNA integrity in all living organisms. Responses to DNA damage include, among others, induction of DNA repair and blocking of cell cycle progression in order to prevent transmission of damaged DNA to daughter cells. Here, we tested the effect of the antibiotic zeocin, inducing double stranded DNA breaks, on the cell cycle of synchronized cultures of the green alga Chlamydomonas reinhardtii. After zeocin application, DNA replication partially occurred but nuclear and cellular divisions were completely blocked. Application of zeocin combined with caffeine, known to alleviate DNA checkpoints, decreased cell viability significantly. This was probably caused by a partial overcoming of the cell cycle progression block in such cells, leading to aberrant cell divisions. The cell cycle block was accompanied by high steady state levels of mitotic cyclin-dependent kinase activity. The data indicate that DNA damage response in C. reinhardtii is connected to the cell cycle block, accompanied by increased and stabilized mitotic cyclin-dependent kinase activity.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cells
ISSN
2073-4409
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
7
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
735
UT code for WoS article
000478902000118
EID of the result in the Scopus database
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