Comparative Integrated Omics Analysis of the Hfq Regulon in Bordetella pertussis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00517771" target="_blank" >RIV/61388971:_____/19:00517771 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1422-0067/20/12/3073" target="_blank" >https://www.mdpi.com/1422-0067/20/12/3073</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms20123073" target="_blank" >10.3390/ijms20123073</a>
Alternative languages
Result language
angličtina
Original language name
Comparative Integrated Omics Analysis of the Hfq Regulon in Bordetella pertussis
Original language description
Bordetella pertussis is a Gram-negative strictly human pathogen of the respiratory tract and the etiological agent of whooping cough (pertussis). Previously, we have shown that RNA chaperone Hfq is required for virulence of B. pertussis. Furthermore, microarray analysis revealed that a large number of genes are affected by the lack of Hfq. This study represents the first attempt to characterize the Hfq regulon in bacterial pathogen using an integrative omics approach. Gene expression profiles were analyzed by RNA-seq and protein amounts in cell-associated and cell-free fractions were determined by LC-MS/MS technique. Comparative analysis of transcriptomic and proteomic data revealed solid correlation (r(2) = 0.4) considering the role of Hfq in post-transcriptional control of gene expression. Importantly, our study confirms and further enlightens the role of Hfq in pathogenicity of B. pertussis as it shows that Delta hfq strain displays strongly impaired secretion of substrates of Type III secretion system (T3SS) and substantially reduced resistance to serum killing. On the other hand, significantly increased production of proteins implicated in transport of important metabolites and essential nutrients observed in the mutant seems to compensate for the physiological defect introduced by the deletion of the hfq gene.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
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Volume of the periodical
20
Issue of the periodical within the volume
12
Country of publishing house
CH - SWITZERLAND
Number of pages
16
Pages from-to
3073
UT code for WoS article
000473756000223
EID of the result in the Scopus database
2-s2.0-85068817952