Diel changes and diversity of pufM expression in freshwater communities of anoxygenic phototrophic bacteria
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00518173" target="_blank" >RIV/61388971:_____/19:00518173 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/19:00518173
Result on the web
<a href="https://www.nature.com/articles/s41598-019-55210-x" target="_blank" >https://www.nature.com/articles/s41598-019-55210-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-019-55210-x" target="_blank" >10.1038/s41598-019-55210-x</a>
Alternative languages
Result language
angličtina
Original language name
Diel changes and diversity of pufM expression in freshwater communities of anoxygenic phototrophic bacteria
Original language description
The anoxygenic phototrophic bacteria (APB) are an active component of aquatic microbial communities. While DNA-based studies have delivered a detailed picture of APB diversity, they cannot provide any information on the activity of individual species. Therefore, we focused on the expression of a photosynthetic gene by APB communities in two freshwater lakes (Cep lake and the Římov Reservoir) in the Czech Republic. First, we analyzed expression levels of pufM during the diel cycle using RT-qPCR. The transcription underwent a strong diel cycle and was inhibited during the day in both lakes. Then, we compared DNA- (total) and RNA-based (active) community composition by sequencing pufM amplicon libraries. We observed large differences in expression activity among different APB phylogroups. While the total APB community in the Římov Reservoir was dominated by Betaproteobacteria, Alphaproteobacteria prevailed in the active library. A different situation was encountered in the oligotrophic lake Cep where Betaproteobacteria (order Burkholderiales) dominated both the DNA and RNA libraries. Interestingly, in Cep lake we found smaller amounts of highly active uncultured phototrophic Chloroflexi, as well as phototrophic Gemmatimonadetes. Despite the large diversity of APB communities, light repression of pufM expression seems to be a common feature of all aerobic APB present in the studied lakes.
Czech name
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Czech description
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Classification
Type
J<sub>ost</sub> - Miscellaneous article in a specialist periodical
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
Dec 2019
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
18766
UT code for WoS article
000501992600001
EID of the result in the Scopus database
2-s2.0-85076431246