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Redox properties of individual quercetin moieties

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00518259" target="_blank" >RIV/61388971:_____/19:00518259 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388955:_____/19:00518259 RIV/61989592:15110/19:73595251

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S0891584919308974?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0891584919308974?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.freeradbiomed.2019.08.001" target="_blank" >10.1016/j.freeradbiomed.2019.08.001</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Redox properties of individual quercetin moieties

  • Original language description

    Quercetin is one of the most prominent and widely studied flavonoids. Its oxidation has been previously investigated only indirectly by comparative analyses of structurally analogous compounds, e.g. dihydroquercetin (taxifolin). To provide direct evidence about the mechanism of quercetin oxidation, we employed selective alkylation procedures for the step-by-step blocking of individual redox active sites, i.e. the catechol, resorcinol and enol C-3 hydroxyls, as represented by newly prepared quercetin derivatives 1-3. Based on the structure-activity relationship (SAR), electrochemical, and computational (density functional theory) studies, we can clearly confirm that quercetin is oxidized in the following steps: the catechol moiety is oxidized first, forming the benzofuranone derivative via intramolecular rearrangement mechanism, therefore the quercetin C-3 hydroxy group cannot be involved in further oxidation reactions or other biochemical processes. The benzofuranone is oxidized subsequently, followed by oxidation of the resorcinol motif to complete the electrochemical cascade of reactions. Derivatization of individual quercetin hydroxyls has a significant effect on its redox behavior, and, importantly, on its antiradical and stability properties, as shown in DPPH/ABTS radical scavenging assays and UV-Vis spectrophotometry, respectively. The SAR data reported here are instrumental for future studies on the oxidation of biologically or technologically important flavonoids and other polyphenols or polyhydroxy substituted aromatics. This is the first complete and direct study mapping redox properties of individual moieties in quercetin structure.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10610 - Biophysics

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Free Radical Biology and Medicine

  • ISSN

    0891-5849

  • e-ISSN

  • Volume of the periodical

    143

  • Issue of the periodical within the volume

    NOV 1 2019

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    12

  • Pages from-to

    240-251

  • UT code for WoS article

    000496132900024

  • EID of the result in the Scopus database

    2-s2.0-85070671514