Production of recombinant soluble dimeric C-type lectin-like receptors of rat natural killer cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F19%3A00521615" target="_blank" >RIV/61388971:_____/19:00521615 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/19:10402748 RIV/00216208:11320/19:10402748
Result on the web
<a href="https://www.nature.com/articles/s41598-019-52114-8" target="_blank" >https://www.nature.com/articles/s41598-019-52114-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-019-52114-8" target="_blank" >10.1038/s41598-019-52114-8</a>
Alternative languages
Result language
angličtina
Original language name
Production of recombinant soluble dimeric C-type lectin-like receptors of rat natural killer cells
Original language description
Working at the border between innate and adaptive immunity, natural killer (NK) cells play a key role in the immune system by protecting healthy cells and by eliminating malignantly transformed, stressed or virally infected cells. NK cell recognition of a target cell is mediated by a receptor 'zipper' consisting of various activating and inhibitory receptors, including C-type lectin-like receptors. Among this major group of receptors, two of the largest rodent receptor families are the NKR-P1 and the Clr receptor families. Although these families have been shown to encode receptor-ligand pairs involved in MHC-independent self-nonself discrimination and are a target for immune evasion by tumour cells and viruses, structural mechanisms of their mutual recognition remain less well characterized. Therefore, we developed a non-viral eukaryotic expression system based on transient transfection of suspension-adapted human embryonic kidney 293 cells to produce soluble native disulphide dimers of NK cell C-type lectin-like receptor ectodomains. The expression system was optimized using green fluorescent protein and secreted alkaline phosphatase, easily quantifiable markers of recombinant protein production. We describe an application of this approach to the recombinant protein production and characterization of native rat NKR-P1B and Clr-11 proteins suitable for further structural and functional studies.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
—
Volume of the periodical
9
Issue of the periodical within the volume
NOV28
Country of publishing house
GB - UNITED KINGDOM
Number of pages
16
Pages from-to
17836
UT code for WoS article
000499236800001
EID of the result in the Scopus database
2-s2.0-85075674332