Insights from enzymatic degradation of cellulose and hemicellulose to fermentable sugars- a review

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00525065" target="_blank" >RIV/61388971:_____/20:00525065 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0961953420300155" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0961953420300155</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.biombioe.2020.105481" target="_blank" >10.1016/j.biombioe.2020.105481</a>

Result language

angličtina

Original language name

Insights from enzymatic degradation of cellulose and hemicellulose to fermentable sugars- a review

Original language description

Lignocellulose, the most abundant and renewable resource on Earth is an important raw material, which can be converted into high value products. However, to this end, it needs to be pretreated physically, chemically, or biologically. Its complex structure and recalcitrance against physical, chemical, or biological degradation render its breakdown an important target of study. The understanding of the enzymatic processes of lignocellulose breakdown and the changes in its chemistry are thus essential. Here, we review the current analytical challenges in the analysis of lignocellulose composition, lignocelluloytic pretreatment, analysis of enzymatic hydrolysis catalyzed by cellulases or hemicellulases and their biotechnological applications.nComplex techniques including biochemical, genomic, and metagenomics methods such as high performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD), Respiration Activity Monitoring System (RAMOS), and next-generation sequencing are described. HPAEC-PAD is a promising, rapid, and reliable analytical technique for sugar quantification following lignocellulose breakdown. RAMOS is an effective technique for monitoring the growth of microorganisms during the different phases of enzyme production, enzymatic hydrolysis, and fermentation. The emergence of high throughput, next-generation sequencing techniques has enriched the databases of genes encoding glycoside hydrolase classes commonly involved in lignocellulose decomposition, and this knowledge can be readily used to analyse the involved processes. Still, novel analytical methods are highly welcome to understand the complete process of lignocelluloytic breakdown. In order to decrease environmental pollution and to save energy, lignocellulose conversion needs to be promoted in order to effectively compete with fossil resources on a global scale in future.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomass & Bioenergy

ISSN

0961-9534

e-ISSN

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Volume of the periodical

134

Issue of the periodical within the volume

MAR

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

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UT code for WoS article

000517497400018

EID of the result in the Scopus database

2-s2.0-85078758628