Isolation of astaxanthin monoesters from the microalgae Haematococcus pluvialis by high performance countercurrent chromatography (HPCCC) combined with high performance liquid chromatography (HPLC)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00525383" target="_blank" >RIV/61388971:_____/20:00525383 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/20:10413836

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S2211926419312688?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2211926419312688?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.algal.2020.101947" target="_blank" >10.1016/j.algal.2020.101947</a>

Result language

angličtina

Original language name

Isolation of astaxanthin monoesters from the microalgae Haematococcus pluvialis by high performance countercurrent chromatography (HPCCC) combined with high performance liquid chromatography (HPLC)

Original language description

Astaxanthin is a potent natural antioxidant with beneficial bioactivities demonstrated primarily for its free (nonesterified) form. However, its natural producer, the microalgae Haematococcus pluvialis synthesizes astaxanthin mostly in ester forms which have been little valorized so far. Hence, to contribute to the commercial use of astaxanthin esters, a scalable and efficient isolation technology is required. In this study, five astaxanthin monoesters were isolated from H. pluvialis using high performance countercurrent chromatography (HPCCC), where the lower phase of a biphasic solvent system (n-heptane:acetonitrile, ratio 5:5, v/v) was used as a mobile phase. Around 200 mg of biomass extract was subjected to the HPCCC leading to a separation of the target astaxanthin esters. To further increase the process productivity, a multi-injection HPCCC method was developed by combining two elution modes (reverse phase and co-current). In co-current elution mode, both the mobile and stationary phases were pumped simultaneously at flow rates of 3 and 1 mL/min respectively, so that the stationary phase that gets lost during each separation cycle is replenished. In total, five injections of samples (200 mg of extract, each) were achieved. Final purification with high performance liquid chromatography (HPLC) afforded five astaxanthin derivatives esterified with α-linolenic acid (1, 4 mg), linoleic acid (2, 8 mg), palmitic acid (3, 8 mg), oleic acid (4, 12 mg) and stearic acid (5, 1 mg) with purities of 98%, as determined by HPLC analysis. Only compound 4 exhibited a cytotoxic effect against human gastric cancer cells. The present study shows a useful approach for obtaining individual astaxanthin esters from H. pluvialis.n

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Algal Research-Biomass Biofuels and Bioproducts

ISSN

2211-9264

e-ISSN

—

Volume of the periodical

49

Issue of the periodical within the volume

AUG 2020

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

101947

UT code for WoS article

000540736500003

EID of the result in the Scopus database

2-s2.0-85085331841