Phospholipid turnover rates suggest that bacterial community growth rates in the open ocean are systematically underestimated

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00531763" target="_blank" >RIV/61388971:_____/20:00531763 - isvavai.cz</a>

Result on the web

<a href="https://aslopubs.onlinelibrary.wiley.com/doi/10.1002/lno.11424" target="_blank" >https://aslopubs.onlinelibrary.wiley.com/doi/10.1002/lno.11424</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/lno.11424" target="_blank" >10.1002/lno.11424</a>

Result language

angličtina

Original language name

Phospholipid turnover rates suggest that bacterial community growth rates in the open ocean are systematically underestimated

Original language description

Heterotrophic bacteria in the surface ocean play a critical role in the global carbon cycle and the magnitude of this role depends on their growth rates. Although methods for determining bacterial community growth rates based on incorporation of radiolabeled thymidine and leucine are widely accepted, they are based on a number of assumptions and simplifications. We sought to independently assess these methods by comparing bacterial growth rates to turnover rates of bacterial membranes using previously published methods in a range of open-ocean settings. We found that turnover rates for heterotrophic bacterial phospholipids averaged 0.80 +/- 0.35 d(-1). This was supported by independent measurements of turnover rates of a membrane-bound pigment in photoheterotrophic bacteria, bacteriochlorophyll a (0.85 +/- 0.09 d(-1)). By contrast, bacterial growth rates measured by uptake of radiolabeled thymidine and leucine were 0.12 +/- 0.08 d(-1), well within the range expected from the literature. We explored whether the discrepancies between phospholipid turnover rates and bacterial growth rate could be explained by membrane recycling/remodeling and other factors, but were left to conclude that the radiolabeled thymidine and leucine incorporation methods substantially underestimated actual bacterial growth rates. We use a simple model to show that the faster bacterial growth rates we observed can be accommodated within the constraints of the microbial carbon budget if bacteria are smaller than currently thought, grow with greater efficiency, or some combination of these two factors.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10617 - Marine biology, freshwater biology, limnology

Project

<a href="/en/project/GA13-11281S" target="_blank" >GA13-11281S: Turning the microbial loop</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Limnology and Oceanography

ISSN

0024-3590

e-ISSN

—

Volume of the periodical

65

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

15

Pages from-to

1876-1890

UT code for WoS article

000521804200001

EID of the result in the Scopus database

2-s2.0-85082413773