Comparative analysis of thylakoid protein complexes in state transition mutantsnsiandstn7: focus on PSI and LHCII
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00532434" target="_blank" >RIV/61388971:_____/20:00532434 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s11120-020-00711-4" target="_blank" >https://link.springer.com/article/10.1007/s11120-020-00711-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11120-020-00711-4" target="_blank" >10.1007/s11120-020-00711-4</a>
Alternative languages
Result language
angličtina
Original language name
Comparative analysis of thylakoid protein complexes in state transition mutantsnsiandstn7: focus on PSI and LHCII
Original language description
The photosynthetic machinery of plants can acclimate to changes in light conditions by balancing light-harvesting between the two photosystems (PS). This acclimation response is induced by the change in the redox state of the plastoquinone pool, which triggers state transitions through activation of the STN7 kinase and subsequent phosphorylation of light-harvesting complex II (LHCII) proteins. Phosphorylation of LHCII results in its association with PSI (state 2), whereas dephosphorylation restores energy allocation to PSII (state 1). In addition to state transition regulation by phosphorylation, we have recently discovered that plants lacking the chloroplast acetyltransferase NSI are also locked in state 1, even though they possess normal LHCII phosphorylation. This defect may result from decreased lysine acetylation of several chloroplast proteins. Here, we compared the composition of wild type (wt),stn7andnsithylakoid protein complexes involved in state transitions separated by Blue Native gel electrophoresis. Protein complex composition and relative protein abundances were determined by LC-MS/MS analyses using iBAQ quantification. We show that despite obvious mechanistic differences leading to defects in state transitions, no major differences were detected in the composition of PSI and LHCII between the mutants. Moreover, bothstn7andnsiplants show retarded growth and decreased PSII capacity under fluctuating light as compared to wt, while the induction of non-photochemical quenching under fluctuating light was much lower in bothnsimutants than instn7.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Photosynthesis Research
ISSN
0166-8595
e-ISSN
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Volume of the periodical
145
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
16
Pages from-to
15-30
UT code for WoS article
000541836000003
EID of the result in the Scopus database
2-s2.0-85078167204