Tracing incorporation of heavy water into proteins for species-specific metabolic activity in complex communities

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00532445" target="_blank" >RIV/61388971:_____/20:00532445 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1874391920301597" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1874391920301597</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jprot.2020.103791" target="_blank" >10.1016/j.jprot.2020.103791</a>

Result language
angličtina
Original language name
Tracing incorporation of heavy water into proteins for species-specific metabolic activity in complex communities
Original language description
Stable isotope probing (SIP) approaches are a suitable tool to identify active organisms in bacterial communities, but adding isotopically labeled substrate can alter both the structure and the functionality of the community. Here, we validated and demonstrated a substrate-independent protein-SIP protocol using isotopically labeled water that captures the entire microbial activity of a community. We found that O-18 yielded a higher incorporation rate into peptides and thus comprised a higher sensitivity. We then applied the method to an in vitro model of a human distal gut microbial ecosystem grown in two medium formulations, to evaluate changes in microbial activity between a high-fiber and high-protein diet. We showed that only little changes are seen in the community structure but the functionality varied between the diets. In conclusion, our approach can detect species-specific metabolic activity in complex bacterial communities and more specifically to quantify the amount of amino acid synthesis. Heavy water makes possible to analyze the activity of bacterial communities for which adding an isotopically labeled energy and nutrient sources is not easily feasible.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10606 - Microbiology

Project
<a href="/en/project/GJ20-02022Y" target="_blank" >GJ20-02022Y: Dawn of the dead: Chemistry and turnover of dead microbes, and their role in the soil food-chain</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)