Toll-Like Receptor 4 Signaling in the Ileum and Colon of Gnotobiotic Piglets Infected with Salmonella Typhimurium or Its Isogenic Deletion rfa Mutants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00534581" target="_blank" >RIV/61388971:_____/20:00534581 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/20:00534581

Result on the web

<a href="https://www.mdpi.com/2072-6651/12/9/545" target="_blank" >https://www.mdpi.com/2072-6651/12/9/545</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/toxins12090545" target="_blank" >10.3390/toxins12090545</a>

Result language

angličtina

Original language name

Toll-Like Receptor 4 Signaling in the Ileum and Colon of Gnotobiotic Piglets Infected with Salmonella Typhimurium or Its Isogenic Deletion rfa Mutants

Original language description

Salmonella Typhimurium is a Gram-negative bacterium that causes enterocolitis in humans and pigs. Lipopolysaccharide (LPS) is a component of the outer leaflet of Gram-negative bacteria that provokes endotoxin shock. LPS can be synthesized completely or incompletely and creates S (smooth) or R (rough) chemotypes. Toll-like receptors (TLR) 2, 4, and 9 initiate an inflammatory reaction to combat bacterial infections. We associated/challenged one-week-old gnotobiotic piglets with wild-type S. Typhimurium with S chemotype or its isogenic increment rfa mutants with R chemotype LPS. The wild-type S. Typhimurium induced TLR2 and TLR4 mRNA expression but not TLR9 mRNA expression in the ileum and colon of one-week-old gnotobiotic piglets 24 h after challenge. The TLR2 and TLR4 stimulatory effects of the S. Typhimurium increment rfa mutants were related to the completeness of their LPS chain. The transcription of IL-12/23 p40, IFN-gamma, and IL-6 in the intestine and the intestinal and plasmatic levels of IL-12/23 p40 and IL-6 but not IFN-gamma were related to the activation of TLR2 and TLR4 signaling pathways. The avirulent S. Typhimurium increment rfa mutants are potentially useful for modulation of the TLR2 and TLR4 signaling pathways to protect the immunocompromised gnotobiotic piglets against subsequent infection with the virulent S. Typhimurium.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30108 - Toxicology

Project

<a href="/en/project/LD15090" target="_blank" >LD15090: A modification of a gut microbiota as a prevention of enteric diseases in newborns: a study in a piglet gnotobiotic model</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Toxins

ISSN

2072-6651

e-ISSN

—

Volume of the periodical

12

Issue of the periodical within the volume

9

Country of publishing house

CH - SWITZERLAND

Number of pages

16

Pages from-to

545

UT code for WoS article

000582451400001

EID of the result in the Scopus database

2-s2.0-85089932287