Gut Microbiome Alters the Activity of Liver Cytochromes P450 in Mice With Sex-Dependent Differences

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00535227" target="_blank" >RIV/61388971:_____/20:00535227 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15110/20:73605109

Result on the web

<a href="https://www.frontiersin.org/articles/10.3389/fphar.2020.01303/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fphar.2020.01303/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fphar.2020.01303" target="_blank" >10.3389/fphar.2020.01303</a>

Result language

angličtina

Original language name

Gut Microbiome Alters the Activity of Liver Cytochromes P450 in Mice With Sex-Dependent Differences

Original language description

Sexual differences and the composition/function of the gut microbiome are not considered the most important players in the drug metabolism field, however, from the recent data it is obvious that they may significantly affect the response of the patient to therapy. Here, we evaluated the effect of microbial colonization and sex differences on mRNA expression and the enzymatic activity of hepatic cytochromes P450 (CYPs) in germ-free (GF) mice, lacking the intestinal flora, and control specific-pathogen-free (SPF) mice. We observed a significant increase in the expression ofCyp3a11in female SPF mice compared to the male group. However, the sex differences were erased in GF mice, and the expression ofCyp3a11was about the same in both sexes. We have also found higherCyp2c38gene expression in female mice compared to male mice in both the SPF and GF groups. Moreover, these changes were confirmed at the level of enzymatic activity, where the female mice exhibit higher levels of functional CYP2C than males in both groups. Interestingly, we observed the same trend as with CYP3A enzymes: a diminished difference between the sexes in GF mice. The presented data indicate that the mouse gut microbiome plays an important role in sustaining sexual dimorphism in terms of hepatic gene expression and metabolism.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30102 - Immunology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Pharmacology

ISSN

1663-9812

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

OCT 2 2020

Country of publishing house

CH - SWITZERLAND

Number of pages

6

Pages from-to

01303

UT code for WoS article

000577726800001

EID of the result in the Scopus database

2-s2.0-85092776545