Multiparametric flow cytometry analysis of peripheral blood B cell trafficking differences among Epstein-Barr virus infected and uninfected subpopulations

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00537110" target="_blank" >RIV/61388971:_____/20:00537110 - isvavai.cz</a>

Result on the web

<a href="https://biomed.papers.upol.cz/artkey/bio-202003-0004_multiparametric-flow-cytometry-analysis-of-peripheral-blood-b-cell-trafficking-differences-among-epstein-barr-v.php" target="_blank" >https://biomed.papers.upol.cz/artkey/bio-202003-0004_multiparametric-flow-cytometry-analysis-of-peripheral-blood-b-cell-trafficking-differences-among-epstein-barr-v.php</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/bp.2019.052" target="_blank" >10.5507/bp.2019.052</a>

Result language

angličtina

Original language name

Multiparametric flow cytometry analysis of peripheral blood B cell trafficking differences among Epstein-Barr virus infected and uninfected subpopulations

Original language description

Aims. Epstein-Barr virus (EBV) targets predominantly B cells and these cells could acquire new phenotype characteristics. Here we analyzed whether EBV-infected and -uninfected B cells from healthy subjects differ in proportion of dominant phenotypes, maturation stage, and homing receptors expression.nMethods. EBV-infected and -uninfected cells were identified by flow cytometry using fluorophore-labeled EBV RNA-specific DNA probes combined with fluorophore-labeled antibody to surface lineage markers, integrins, chemokine receptors, and immunoglobulin isotypes, including intracellular ones.nResults. Our results show that the trafficking characteristics of EBERpos B cells are distinct from EBERneg B cells with most dominant differences detected for alpha 4 beta 1 and alpha 4 beta 7 and CCR5 and CCR7. EBV-positive cells are predominantly memory IgM(+) B cells or plasmablasts/plasma cells (PB/PC) positive for IgA or less for IgM. In comparison to uninfected B cells, less EBV-positive B cells express alpha 4 beta 7 and almost no cells express alpha 4 beta 1. EBV-positive B cells contained significantly higher proportion of CCR5(+) and CCR7(+) cells in comparison to EBV-negative cells. In vitro exposure of blood mononuclear cells to pro-inflammatory cytokine IL-6 reduces population of EBV-positive B cell.nConclusion. Although EBV-infected B cells represent only a minor subpopulation, their atypical functions could contribute in predisposed person to development abnormities such as some autoimmune diseases or tumors. Using multi-parameter flow cytometry we characterized differences in migration of EBV-positive and -negative B cells of various maturation stage and isotype of produced antibodies particularly different targeting to mucosal tissues of gastrointestinal and respiratory tracts.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/NV15-33686A" target="_blank" >NV15-33686A: The study of the relation between Epstein-Barr virus infection and development of IgA nephropathy</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BIOMEDICAL PAPERS-OLOMOUC

ISSN

1804-7521

e-ISSN

—

Volume of the periodical

164

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

247-254

UT code for WoS article

000595645600004

EID of the result in the Scopus database

2-s2.0-85086109490