Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00537296" target="_blank" >RIV/61388971:_____/20:00537296 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/20:10422992
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S2211124720315230" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2211124720315230</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.celrep.2020.108534" target="_blank" >10.1016/j.celrep.2020.108534</a>

Result language
angličtina
Original language name
Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts
Original language description
Canonical mRNA translation in eukaryotes begins with the formation of the 43S pre-initiation complex (PIC). Its assembly requires binding of initiator Met-tRNAiMet and several eukaryotic initiation factors (eIFs) to the small ribosomal subunit (40S). Compared to their mammalian hosts, trypanosomatids present significant structural differences in their 40S, suggesting substantial variability in translation initiation. Here, we determine the structure of the 43S PIC from Trypanosoma cruzi, the parasite causing Chagas disease. Our structure shows numerous specific features, such as the variant eIF3 structure and its unique interactions with the large rRNA expansion segments (ESs) 9S, 7S, and 6S, and the association of a kinetoplastid-specific DDX60-like helicase. It also reveals the 40S-binding site of the eIF5 C-terminal domain and structures of key terminal tails of several conserved eIFs underlying their activities within the PIC. Our results are corroborated by glutathione S-transferase (GST) pull-down assays in both human and T. cruzi and mass spectrometry data.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10606 - Microbiology

Project
<a href="/en/project/GX19-25821X" target="_blank" >GX19-25821X: Global and transcript-specific analysis of translational control in disease.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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