Mutual interactions of E. andrei earthworm and pathogens during the process of vermicomposting

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00539798" target="_blank" >RIV/61388971:_____/20:00539798 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/20:84387

Result on the web

<a href="https://link.springer.com/article/10.1007/s11356-019-04329-5" target="_blank" >https://link.springer.com/article/10.1007/s11356-019-04329-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11356-019-04329-5" target="_blank" >10.1007/s11356-019-04329-5</a>

Result language

angličtina

Original language name

Mutual interactions of E. andrei earthworm and pathogens during the process of vermicomposting

Original language description

Vermicomposting is a process by which earthworms together with microorganisms degrade organic wastes into a humus-like material called vermicompost. This process does not include a thermophilic stage, and therefore, the possible presence of pathogens represents a potential health hazard. To elucidate the effect of earthworms in the selective reduction of pathogens, grape marc substrate was artificially inoculated with Escherichia coli, Enterococcus spp., thermotolerant coliform bacteria (TCB), and Salmonella spp., and their reduction during vermicomposting was monitored. Various defense mechanisms eliminating microorganisms in the earthworm gut were assumed to be involved in the process of pathogen reduction. Therefore, we followed the expression of three pattern recognition receptors (coelomic cytolytic factor (CCF), lipopolysaccharide-binding protein (LBP), and Toll-like receptor (v-TLR)), two antimicrobial molecules (fetidin/lysenins and lysozyme), and heat shock protein HSP70. We detected the significant decrease of some defense molecules (fetidin/lysenins and LBP) in all pathogen-inoculated substrates, and the increase of CCF and LBP in the Salmonella spp.-inoculated substrate. At the same time, the reduction of pathogens during vermicomposting was assessed. We observed the accelerated reduction of E. coli, Enterococcus spp., and TCB in pathogen-inoculated substrates with earthworms compared to that without earthworms. Moreover, the differences between the microbiome of grape marc substrate and earthworm intestines were determined by high throughput sequencing. This analysis revealed that the bacterial composition of grape marc substrate differed from the composition of the content of earthworm intestines, suggesting the elimination of specific bacterial species during food passage through the gut.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/QJ1530034" target="_blank" >QJ1530034: Legislative footings for greater application of composts, especially vermicompost, on agricultural land</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Environmental Science and Pollution Research

ISSN

0944-1344

e-ISSN

—

Volume of the periodical

27

Issue of the periodical within the volume

27

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

33429-33437

UT code for WoS article

000559349600005

EID of the result in the Scopus database

2-s2.0-85062775611